Publication: The use of the replica technique to record fluid emerging from exposed dentine
Issued Date
2004-08-01
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ISSN
00039969
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2-s2.0-2942715339
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Oral Biology. Vol.49, No.8 (2004), 613-619
Suggested Citation
V. Kerdvongbundit, S. Thiradilok, N. Vongsavan, B. Matthews The use of the replica technique to record fluid emerging from exposed dentine. Archives of Oral Biology. Vol.49, No.8 (2004), 613-619. doi:10.1016/j.archoralbio.2003.11.009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21298
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Title
The use of the replica technique to record fluid emerging from exposed dentine
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Abstract
The effect of pulpal cavity pressure on structures present on etched and unetched dentine was determined in 24 human premolars in vitro. The dentine was exposed in vivo. After extraction, the pulp was removed and the crown stored in 2.5 N sodium hydroxide for 3 days to remove the odontoblasts. The pulp cavity was filled with Ringer's solution at atmospheric pressure, +30 or -5 mmHg. After 30 s-5 min a silicone-rubber impression was taken of the cavity floor and a resin cast prepared and examined in a SEM. The replicas reproduced the dentine surface accurately when there was no outward flow through dentine. With a pressure of + 30 mmHg in the pulp cavity, but not at the lower pressures, droplets were present on unetched dentine after 30 s. Droplets accumulated less rapidly on etched dentine, indicating that it may act as a sponge. © 2004 Elsevier Ltd. All rights reserved.