Publication: Cytotoxicity evaluation of gutta-percha solvents: Chloroform and GP-Solvent (limonene)
Issued Date
2004-12-01
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ISSN
10792104
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2-s2.0-9944231413
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Mahidol University
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SCOPUS
Bibliographic Citation
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. Vol.98, No.6 (2004), 756-759
Suggested Citation
La Ongthong Vajrabhaya, Suwanna K. Suwannawong, Roongrawee Kamolroongwarakul, Lalita Pewklieng Cytotoxicity evaluation of gutta-percha solvents: Chloroform and GP-Solvent (limonene). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. Vol.98, No.6 (2004), 756-759. doi:10.1016/j.tripleo.2004.05.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21296
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Title
Cytotoxicity evaluation of gutta-percha solvents: Chloroform and GP-Solvent (limonene)
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Abstract
Objective The purpose of this study was to compare the cytotoxicity of 2 gutta-percha solvents, chloroform and GP-Solvent, on cell line L929. Study design 2 gutta-percha solvents were diluted into the concentrations of 1:100, 1:400, and 1:800. The experiment was done in a 96-well tissue-culture plate. Cell viability of L929 was determined after each gutta-percha solvent was left in contact with MTT solution for 3 hours. Results Both solvents proved toxic at the same levels of concentrations of 1:100 and 1:400 (P>.05). At the dilution of 1:800 the GP-Solvent seems to be more toxic than the chloroform (P < .05). Conclusions Within the limitation of this experiment, GP-Solvent was not less cytotoxic than chloroform to the target cells. Because in clinical procedures we use a higher concentration of solvent to dissolve gutta-percha for retreatment than that used in this study, the overflowing of liquefied gutta-percha, or solvent out of apical foramen, should be a cause for concern.