Publication: Multilocus phylogenetic analyses, pullulan production and xylanase activity of tropical isolates of Aureobasidium pullulans
7
Issued Date
2009-10-01
Resource Type
ISSN
14698102
09537562
09537562
Other identifier(s)
2-s2.0-70350564179
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Mahidol University
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SCOPUS
Bibliographic Citation
Mycological Research. Vol.113, No.10 (2009), 1107-1120
Suggested Citation
Pennapa Manitchotpisit, Timothy D. Leathers, Stephen W. Peterson, Cletus P. Kurtzman, Xin Liang Li, Douglas E. Eveleigh, Pongtharin Lotrakul, Sehanat Prasongsuk, Christopher A. Dunlap, Karl E. Vermillion, Hunsa Punnapayak Multilocus phylogenetic analyses, pullulan production and xylanase activity of tropical isolates of Aureobasidium pullulans. Mycological Research. Vol.113, No.10 (2009), 1107-1120. doi:10.1016/j.mycres.2009.07.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/26975
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Title
Multilocus phylogenetic analyses, pullulan production and xylanase activity of tropical isolates of Aureobasidium pullulans
Abstract
Aureobasidium pullulans is the source of the commercially valuable polysaccharide pullulan and the enzyme xylanase. Isolates are typically off-white to pale pink or black on solid media, while some tropical isolates have been described as 'color variants' with bright pigments of red, yellow or purple. We sequenced 5 loci (internal transcribed spacer, intergenic spacer 1, translation elongation factor-1 alpha, beta tubulin, and RNA polymerase II) from 45 new isolates from Thailand. Based on the phylogenetic analyses, isolates were classified into 12 clades. Each clade showed different colors on different culture media including two clades with 'color variants' and some clades exhibited high levels of pullulan production or xylanase activity. Colony characteristics do not correlate perfectly with DNA sequence phylogeny or the physiological characters, but DNA sequence differences rapidly identify isolates with genetic novelty. © 2009 The British Mycological Society.