Publication: Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia
Issued Date
2016-03-01
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ISSN
19326203
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2-s2.0-84962159027
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.11, No.3 (2016)
Suggested Citation
Mohd Ridzuan Mohd Abd Razak, Umi Rubiah Sastu, Nor Azrina Norahmad, Abass Abdu-Karim, Amirrudin Muhammad, Prem Kumar Muniandy, Jenarun Jelip, Christina Rundi, Mallika Imwong, Rose Nani Mudin, Noor Rain Abdullah Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia. PLoS ONE. Vol.11, No.3 (2016). doi:10.1371/journal.pone.0152415 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40648
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Title
Genetic diversity of plasmodium falciparum populations in malaria declining areas of Sabah, East Malaysia
Abstract
© Copyright: 2016 Mohd Abd Razak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Malaysia has a national goal to eliminate malaria by 2020. Understanding the genetic diversity of malaria parasites in residual transmission foci can provide invaluable information which may inform the intervention strategies used to reach elimination targets. This study was conducted to determine the genetic diversity level of P. falciparum isolates in malaria residual foci areas of Sabah. Malaria active case detection was conducted in Kalabakan and Kota Marudu. All individuals in the study sites were screened for malaria infection by rapid diagnostic test. Blood from P. falciparum-infected individuals were collected on filter paper prior to DNA extraction. Genotyping was performed using merozoite surface protein-1 (MSP-1), merozoite surface protein-2 (MSP-2), glutamate rich protein (GLURP) and 10 neutral microsatellite loci markers. The size of alleles, multiplicity of infection (MOI), mean number of alleles (Na), expected heterozygosity (He), linkage disequilibrium (LD) and genetic differentiation (FST) were determined. In Kalabakan, the MSP-1 and MSP-2 alleles were predominantly K1 and FC27 family types, respectively. The GLURP genotype VI (751-800 bp) was predominant. The MOI for MSP-1 and MSP-2 were 1.65 and 1.20, respectively. The Na per microsatellite locus was 1.70. The He values for MSP-1, MSP-2, GLURP and neutral microsatellites were 0.17, 0.37, 0.70 and 0.33, respectively. In Kota Marudu, the MSP-1 and MSP-2 alleles were predominantly MAD20 and 3D7 family types, respectively. The GLURP genotype IV (651-700 bp) was predominant. The MOI for both MSP-1 and MSP-2 was 1.05. The Na per microsatellite locus was 3.60. The He values for MSP-1, MSP-2, GLURP and neutral microsatellites were 0.24, 0.25, 0.69 and 0.30, respectively. A significant LD was observed in Kalabakan (0.495, p<0.01) and Kota Marudu P. falciparum populations (0.601, p<0.01). High genetic differentiation between Kalabakan and Kota Marudu P. falciparum populations was observed (FST= 0.532). The genetic data from the present study highlighted the limited diversity and contrasting genetic pattern of P. falciparum populations in the malaria declining areas of Sabah.