Activation of lucidin‐3‐O‐primveroside mutagenicity by hesperidinase

Abstract

At the maximum dose tested, 100 μg/plate, lucidin‐3‐O‐primveroside (LUC‐Prv) exhibited mutagenic potential in Salmonella typhimurium TA100 without S9 mix but not with the addition of this preparation. When the pH of the tested system was reduced from 7.4 to 6.5, the same results were obtained. However, after hesperidinase treatment at pH 6.5, higher mutagenicity was demonstrated, and the mutagenicity of the hydrolysis product in TA100 decreased in the presence of S9 mix. When the preincubation time of the mixture was kept longer, i.e. 30, 60 and 120 min, the number of revertants in TA100 without S9 mix became higher. When the concentration of hesperidinase was varied from 5 × 10−5 to 2.5 × 10−2 unit, no change in the number of revertants was observed. In contrast, LUC‐Prv and its hydrolysis products demonstrated no mutagenicity in TA98 by the same tested system. Copyright © 1995 John Wiley & Sons, Ltd.