Publication: Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice
Issued Date
2005-03-10
Resource Type
ISSN
01674781
Other identifier(s)
2-s2.0-20044382215
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochimica et Biophysica Acta - Gene Structure and Expression. Vol.1727, No.3 (2005), 151-161
Suggested Citation
Valérie Pujade-Renaud, Christine Sanier, Laurence Cambillau, Arokiaraj Pappusamy, Heddwyn Jones, Natsuang Ruengsri, Didier Tharreau, Hervé Chrestin, Pascal Montoro, Jarunya Narangajavana Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice. Biochimica et Biophysica Acta - Gene Structure and Expression. Vol.1727, No.3 (2005), 151-161. doi:10.1016/j.bbaexp.2004.12.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16368
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Title
Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice
Abstract
The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate useful promoters to drive transgene expression in genetically engineered rubber tree. Four different full length genes were cloned by library screening and a fifth, a partial gene, by adaptor-anchored PCR. Sequence alignment revealed that hevein genes, although highly conserved in their transcribed region, diverged in two groups, with major differences in their promoter region, suggesting a more rapid evolution of the upstream regulatory functions of the genes than the downstream functions of their protein products. The promoter regions from two hevein genes representative of each group were isolated and analyzed in rice. Although both were functional, only the longest promoter sequence (PHev2.1) conferred a high level of expression to the transgene in various tissues of this heterologous host. It was in addition up-regulated by mechanical wounding and fungal infection in leaves. A number of potential cis-regulatory elements were identified in silico and are discussed in view of the expression profiles observed in rice. © 2004 Elsevier B.V. All rights reserved.