Publication: The effect of light-emitting diode irradiation at different wavelengths on calcification of osteoblast-like cells in 3D culture
Issued Date
2015-11-04
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1557170X
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2-s2.0-84953329499
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Mahidol University
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SCOPUS
Bibliographic Citation
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol.2015-November, (2015), 3513-3516
Suggested Citation
Rochaya Chintavalakorn, Tatsanee Tanglitanont, Anak Khantachawana, Kwanchanok Viravaidya-Pasuwat, Peerapong Santiwong The effect of light-emitting diode irradiation at different wavelengths on calcification of osteoblast-like cells in 3D culture. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol.2015-November, (2015), 3513-3516. doi:10.1109/EMBC.2015.7319150 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35798
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Title
The effect of light-emitting diode irradiation at different wavelengths on calcification of osteoblast-like cells in 3D culture
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Abstract
© 2015 IEEE. This study aimed to investigate the effect of four different light-emitting diode (LED) wavelengths on calcification and proliferation of osteoblast-like cells in vitro. MC3T3-E1 cells were seeded within three-dimensional collagen scaffolds and irradiated daily by LED light with peak emission wavelengths of 630-, 680-, 760- and 830-nm at constant fluency of 3.1 J/cm2(irradiance intensity 2 mW/cm2). Cultures were measured for calcium content at day 0, 7, 14, 21, 28, 35 and 42. The significant enhancement in calcium content was observed at the early stage of culture (days 7 and 14) (p<0.05). After that, the calcium content of irradiated groups was similar to that of the controls group. This suggests the transient effect of light irradiation on osteoblastic cell calcification. Only 680-nm irradiated samples revealed a significant enhancement of calcium content until the late stages of culture (from days 21 to 42) (p<0.001). The cyclin D mRNA expression that was investigated 3 hours after stimulation at day3 also show that the 680-nm LED irradiation can enhance cyclin D expression more than others. For enhancing bone mineralization, LED irradiation at the 680-nm is more effective than those at 630-, 760- and 830-nm. Further studies should be investigated in order to obtain the most effective parameters of LLLI on bone regeneration in clinical setting.
