Publication: Rapid influenza a antigen detection using carbon nanostrings as label for lateral flow immunochromatographic assay
Issued Date
2013-12-01
Resource Type
ISSN
19449380
19449399
19449399
Other identifier(s)
2-s2.0-84894203806
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Proceedings of the IEEE Conference on Nanotechnology. (2013), 166-169
Suggested Citation
Natpapas Wiriyachaiporn, Hathainan Sirikett, Tararaj Dharakul Rapid influenza a antigen detection using carbon nanostrings as label for lateral flow immunochromatographic assay. Proceedings of the IEEE Conference on Nanotechnology. (2013), 166-169. doi:10.1109/NANO.2013.6720979 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31460
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Rapid influenza a antigen detection using carbon nanostrings as label for lateral flow immunochromatographic assay
Other Contributor(s)
Abstract
In this study, we investigated a feasibility of using carbon nanostrings as reporters in developing a lateral flow immunochromatographic system for a rapid influenza A antigen detection as a model. Carbon nanostrings were successfully conjugated to monoclonal antibody specific to the nucleoprotein, one of the most abundant influenza A protein, for a signal visualization. To demonstrate the feasibility of the lateral flow immunochromatographic system using carbon nanostrings as reporters for influenza A antigen detection, the system was applied to detect the antigen in an allantoic fluid infected with pandemic 2009 influenza A H1N1 virus. It was shown that the system was able to detect the influenza A nucleoprotein in a complex biological fluid such as an infected allantoic fluid containing 2.8×103 TCID50/mL (50% tissue culture infectious dose). By using carbon nanostrings as reporters combined with lateral flow immunoassay as the mean of antigen detection, a specific recognition of influenza A antigen could be achieved within 15 min. Compared to the existing methods of influenza A diagnosis such as viral isolation and serological approaches, this simple one-step immunochromatographic system avoids laborious and multiple incubation steps, as well as maintaining a sensitive and specific detection which could be useful for developing a point-of-care diagnostic test in other applications. © 2013 IEEE.