Publication: Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis
Issued Date
1995-01-01
Resource Type
ISSN
1574695X
09288244
09288244
Other identifier(s)
2-s2.0-0028819667
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Immunology and Medical Microbiology. Vol.12, No.3-4 (1995), 259-264
Suggested Citation
Le Van Phung, Yuki Han, Shiro Oka, Hisako Hotta, Michael D. Smith, Prapit Theeparakun, Eiko Yabuuchi, Ikuya Yano Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis. FEMS Immunology and Medical Microbiology. Vol.12, No.3-4 (1995), 259-264. doi:10.1016/0928-8244(95)00068-X Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17335
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis
Abstract
The serodiagnosis of melioidosis is commonly performed with tests using protein or polysaccharide as antigen. However, due to the low sensitivity, specificity and difficulty in the preparation of the antigens, more simple, precise and reproducible diagnostic tests were required. A purified glycolipid antigen (GL) which is a specific lipid component of Burkholderia pseudomallei has been used in an ELISA. With this antigen, specific immunoglobulin G (IgG) was detected in 49 out of 50 melioidosis sera. IgG was also detected in 2 out of 185 (Japanese) and 16 out of 181 (Vietnamese) control sera. Thus, the sensitivity was 98.0%, and specificity was 98.9% and 91.1% in the Japanese and Vietnamese sera, respectively. When the ELISA and indirect haemagglutination (IHA) tests were combined, a sensitivity of 100% and specificity of 97.8% were achieved. The advantages of the glycolipid antigen are ease of preparation, stability, high sensitivity and specificity. © 1995.