Publication: Effects of vitamin E deficiency on the activities of lipid-requiring enzymes in rabbit liver and muscle
Issued Date
1979-12-01
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ISSN
00223166
Other identifier(s)
2-s2.0-0018770513
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Nutrition. Vol.109, No.5 (1979), 848-855
Suggested Citation
C. Vajanamarhutue, P. Wilairat, P. Komaratat Effects of vitamin E deficiency on the activities of lipid-requiring enzymes in rabbit liver and muscle. Journal of Nutrition. Vol.109, No.5 (1979), 848-855. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13246
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Title
Effects of vitamin E deficiency on the activities of lipid-requiring enzymes in rabbit liver and muscle
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Abstract
The effects of vitamin E deficiency on membrane integrity were studied by examining the temperature dependence of membrane-bound enzyme activities in liver mitochondria and microsome and in muscle sarcoplasmic reticulum. In vitamin E-deficient rabbits, the specific activities at 37°C of mitochondrial oligomycin-sensitive ATPase (EC 3.6.1.3), β-hydroxybutyrate dehydrogenase (EC 1.1.1.30), and microsomal glucose-6-phosphatase (EC 3.1.3.9) were increased, whereas those of microsomal NADH cytochrome C reductase (EC 1.6.99.3) and sarcoplasmic reticulum Ca-ATPase were reduced in comparison to control rabbits. Arrhenius plots of activity against temperature yielded a linear plot over the range 10 to 40°C in the case of β-hydroxybutyrate dehydrogenase, NADH cytochrome C reductase and oligomycin-sensitive ATPase. In control rabbits, all five enzymes showed a single discontinuity in the Arrhenius plot over the range 16 to 19°C. These results reflect changes to the microenvironment of membrane-bound enzymes as a consequence of vitamin E depletion.