Publication: p53 status and human papillomavirus infection in thai women with cervical carcinoma
Issued Date
2000-03-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0034156899
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.31, No.1 (2000), 66-71
Suggested Citation
Temduang Limpaiboon, Jureerut Pooart, Parvapan Bhattarakosol, Somchai Niruthisard, Wasun Chantratita, Viraphong Lulitanond p53 status and human papillomavirus infection in thai women with cervical carcinoma. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.31, No.1 (2000), 66-71. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26273
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
p53 status and human papillomavirus infection in thai women with cervical carcinoma
Other Contributor(s)
Abstract
Loss of p53 function has been implicated in a wide variety of human malignacies. Many studies suggest that in cervical carcinoma p53 function is inactivated either by gene mutation or by complex formation with E6 oncoprotein product of high-risk human papillomavirus (HPV). The aim of this study was to determine the status of HPV infection and p53 gene mutation as well as their correlation in cervical carcinomas. Formalin-fixed paraffin-embedded tissues of 12 cervicitis, 21 cervical intraepithelial neoplasia grade 3 (CIN 3) and 17 squamous cell carcinomas were determined for the presence of HPV using polymerase chain reaction (PCR) amplification and dot blot hybridization. The status of p53 mutations in exons 5-8 was evaluated by polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) and confirmed by direct nucleotide sequencing. HPV infections were detected in all CIN 3 and squamous cell carcinomas (100%). Mutations of p53 were present in 3 of 38 HPV-positive samples: one with an ATG→ TTC transversion (Met→Leu) in codon 237 of exon 7; and the others with a TGC→TGG transversion (Cys→Trp) in codon 242 of exon 7, and a CGT→CCT transversion (Arg→Pro) in codon 273 of exon 8, respectively. Our findings show that the frequency of p53 mutation is low in primary cervical carcinoma and that the p53 gene mutation and HPV infection are not mutually exclusive events in the development of cervical cancer. Thus, other genetic events independent of p53 inactivation may also significantly contribute to the carcinogenesis of the uterine cervix.