Publication: In vitro inhibitory effects of moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes
Issued Date
2013-01-01
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ISSN
15559475
1934578X
1934578X
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2-s2.0-84887466927
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Mahidol University
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SCOPUS
Bibliographic Citation
Natural Product Communications. Vol.8, No.11 (2013), 1559-1561
Suggested Citation
Boonyadist Vongsak, Wandee Gritsanapan, Yuvadee Wongkrajang, Ibrahim Jantan In vitro inhibitory effects of moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes. Natural Product Communications. Vol.8, No.11 (2013), 1559-1561. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31114
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Title
In vitro inhibitory effects of moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes
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Abstract
The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3-O-glucoside and kaempferol 3-Oglucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3-O-glucoside, crypto-chlorogenic acid, and kaempferol 3-Oglucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 μM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 μM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components.