Publication:
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions

dc.contributor.authorKanoot Jaruthamsophonen_US
dc.contributor.authorThanya Sripoen_US
dc.contributor.authorChonlaphat Sukasemen_US
dc.contributor.authorPornprot Limpraserten_US
dc.contributor.otherPrince of Songkla Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-11T01:59:54Z
dc.date.accessioned2019-03-14T08:01:43Z
dc.date.available2018-12-11T01:59:54Z
dc.date.available2019-03-14T08:01:43Z
dc.date.issued2016-05-01en_US
dc.description.abstract© 2016 Jaruthamsophon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Currently, there are three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions in Asian populations. To analyze available HLA-B∗15:02 screening methods, we compared four screening methods, including a multiplex PCR method, a nested PCR method, a LAMP method and our new in-house PCR-dot blot hybridization method. These methods were reviewed regarding their sensitivity, specificity, false positivity and technical considerations. Possible false positive (FP) alleles and genotypes were checked regarding the primers and probes designs, using the IMGT/HLA database. Expected FP rates in Asian populations were predicted using the Allele Frequencies Net Database. All methods had a sensitivity of more than 99.9%, although giving FP results to certain very rare alleles and genotypes. The multiplex PCR method was the only test that gave FP results to certain genotypes of HLA-B∗15:13, the allele which is prevalent in Southeast Asian populations. In conclusion, the nested PCR, LAMP and our in-house methods could be applied in various Asian populations, but the multiplex PCR, or any test with FP to HLA-B∗15:13, should be applied with caution in the Southeast Asian populations.en_US
dc.identifier.citationPLoS ONE. Vol.11, No.5 (2016)en_US
dc.identifier.doi10.1371/journal.pone.0155907en_US
dc.identifier.issn19326203en_US
dc.identifier.other2-s2.0-84973632360en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/40804
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84973632360&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleComparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactionsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84973632360&origin=inwarden_US

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