Publication: The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies
Issued Date
2002-12-01
Resource Type
ISSN
01251562
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2-s2.0-0043202770
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.33, No.4 (2002), 862-868
Suggested Citation
Somchai Sangkitporn, Parichat Pung-amritt, Siripakorn K. Sangkitporn, Areerat Sangnoi, Voravarn S. Tanphaichitr The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.33, No.4 (2002), 862-868. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20292
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Title
The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies
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Abstract
Accurate and precise hemoglobin separation and the quantitation of Hb A2 and Hb F are essential for the diagnosis of the thalassemias and hemoglobinopathies. Presented in this study is the validation of the the Hb A2 assay of the HbGold analyzer, a fully automated liquid chromatography system for hemoglobin separation and quantitation. Variability of Hb A2 quantitation was quite low; the CV's of within-run, between-run and interlaboratory studies were 1.8-3.1%, 3.4-6.0% and 6.8-8.8% respectively. The results of the %Hb A2 quantitated by HbGold analyzer correlated well with those given by the Bio-Rad Variant Hb testing system (r=0.98). The application of the HbGold analyzer for the diagnosis of the thalassemia phenotypes frequently observed in Thailand is considered. In conclusion, the Hb A2 assay of the HbGold analyzer could be used for the quantitation of Hb A2 and Hb F and the presumptive identification of abnormal hemoglobins.