Publication: Assessment and comparative analysis of a rapid diagnostic test (Tubex®) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania
Accepted Date
2011-05-24
Issued Date
2011-05-24
Copyright Date
2011
Resource Type
Language
eng
ISSN
1471-2334 (electronic)
Rights
Mahidol University
Rights Holder(s)
BioMed Central
Bibliographic Citation
Ley B, Thriemer K, Ame SM, Mtove GM, von Seidlein L, Amos B. Assessment and comparative analysis of a rapid diagnostic test (Tubex®) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania. BMC Infect Dis. 2011 May 24;11:147.
Suggested Citation
Ley, Benedikt, Thriemer, Kamala, Ame, Shaali M., Mtove, George M., von Seidlein, Lorenz, Amos, Ben, Hendriksen, Ilse CE, Mwambuli, Abraham, Shoo, Aikande, Kim, Deok R., Ochiai, Leon R., Favorov, Michael, Clemens, John D., Wilfing, Harald, Deen, Jacqueline L., Ali, Said M. Assessment and comparative analysis of a rapid diagnostic test (Tubex®) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania. Ley B, Thriemer K, Ame SM, Mtove GM, von Seidlein L, Amos B. Assessment and comparative analysis of a rapid diagnostic test (Tubex®) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania. BMC Infect Dis. 2011 May 24;11:147.. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/738
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Title
Assessment and comparative analysis of a rapid diagnostic test (Tubex®) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania
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Abstract
BACKGROUND: Typhoid fever remains a significant health problem in many developing
countries. A rapid test with a performance comparable to that of blood culture
would be highly useful. A rapid diagnostic test for typhoid fever, Tubex®, is
commercially available that uses particle separation to detect immunoglobulin M
directed towards Salmonella Typhi O9 lipopolysaccharide in sera.
METHODS: We assessed the sensitivity and specificity of the Tubex test among
Tanzanian children hospitalized with febrile illness using blood culture as gold
standard. Evaluation was done considering blood culture confirmed S. Typhi with
non-typhi salmonella (NTS) and non - salmonella isolates as controls as well as
with non-salmonella isolates only.
RESULTS: Of 139 samples tested with Tubex, 33 were positive for S. Typhi in blood
culture, 49 were culture-confirmed NTS infections, and 57 were other
non-salmonella infections. Thirteen hemolyzed samples were excluded. Using all
non - S. Typhi isolates as controls, we showed a sensitivity of 79% and a
specificity of 89%. When the analysis was repeated excluding NTS from the pool of
controls we showed a sensitivity of 79% and a specificity of 97%. There was no
significant difference in the test performance using the two different control
groups (p > 0.05).
CONCLUSION: This first evaluation of the Tubex test in an African setting showed
a similar performance to those seen in some Asian settings. Comparison with the
earlier results of a Widal test using the same samples showed no significant
difference (p > 0.05) for any of the performance indicators, irrespective of the
applied control group.