Publication: Microparticle and anti-influenza activity in human respiratory secretion
Issued Date
2017-08-01
Resource Type
ISSN
19326203
Other identifier(s)
2-s2.0-85028468834
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE. Vol.12, No.8 (2017)
Suggested Citation
Ornpreya Suptawiwat, Kanyarat Ruangrung, Chompunuch Boonarkart, Pilaipan Puthavathana, Kittipong Maneechotesuwan, Komgrid Charngkaew, Nusara Chomanee, Prasert Auewarakul Microparticle and anti-influenza activity in human respiratory secretion. PLoS ONE. Vol.12, No.8 (2017). doi:10.1371/journal.pone.0183717 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41408
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Microparticle and anti-influenza activity in human respiratory secretion
Other Contributor(s)
Abstract
© 2017 Suptawiwat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Respiratory secretions, such as saliva and bronchoalveolar fluid, contain anti-influenza activity. Multiple soluble factors have been described that exert anti-influenza activity and are believed to be responsible for the anti-influenza activity in respiratory secretions. It was previously shown that a bronchial epithelial cell culture could produce exosome-like particles with anti-influenza activity. Whether such extracellular vesicles in respiratory secretions have anti-influenza activity is unknown. Therefore, we characterized bronchoalveolar lavage fluid and found microparticles, which mostly stained positive for epithelial cell markers and both a2,3- and a2,6-linked sialic acid. Microparticles were purified from bronchoalveolar lavage fluid and shown to exhibit anti-influenza activity by a hemagglutination inhibition (HI) assay and a neutralization (NT) assay. In addition, physical binding between influenza virions and microparticles was demonstrated by electron microscopy. These findings indicate that respiratory microparticles containing viral receptors can exert anti-viral activity by probably trapping viral particles. This innate mechanism may play an important role in the defense against respiratory viruses.