Publication: A sulfated galactans supplemented diet enhances the expression of immune genes and protects against Vibrio parahaemolyticus infection in shrimp
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Issued Date
2017-06-01
Resource Type
ISSN
10959947
10504648
10504648
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2-s2.0-85018757135
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Mahidol University
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SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.65, (2017), 186-197
Suggested Citation
Tawut Rudtanatip, Nantavadee Boonsri, Somluk Asuvapongpatana, Boonsirm Withyachumnarnkul, Kanokpan Wongprasert A sulfated galactans supplemented diet enhances the expression of immune genes and protects against Vibrio parahaemolyticus infection in shrimp. Fish and Shellfish Immunology. Vol.65, (2017), 186-197. doi:10.1016/j.fsi.2017.04.020 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41482
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Title
A sulfated galactans supplemented diet enhances the expression of immune genes and protects against Vibrio parahaemolyticus infection in shrimp
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Abstract
© 2017 Elsevier Ltd A sulfated galactans (SG) supplemented diet was evaluated for the potential to stimulate immune activity in shrimp Penaeus vannamei (P. vannamei). Shrimp given the SG supplemented diet (0.5, 1 and 2% w/w) for 7 days showed enhanced expression of the downstream signaling mediator of lipopolysaccharide and β-1,3-glucan binding protein (LGBP) and immune related genes including p-NF-κB, IMD, IKKβ and IKKε, antimicrobial peptide PEN-4, proPO-I and II. Following immersion with Vibrio parahaemolyticus (V. parahaemolyticus) for 14 days, the shrimp given the SG supplemented diet (1 and 2% w/w) showed a decrease in bacterial colonies and bacterial toxin gene expression, compared to shrimp given a normal diet, and they reached 50% mortality at day 14. However, shrimp given the normal diet and challenged with the bacteria reached 100% mortality at day 6. SG-fed shrimp increased expression of immune genes related to LGBP signaling at day 1 after the bacterial immersion compared to control (no immersion), which later decreased to control levels. Shrimp on the normal diet also increased expression of immune related genes at day 1 after immersion which however decreased below control levels by day 3. Taken together, the results indicate the efficacy of the SG supplemented diet to enhance the immune activity in shrimp which could offer protection from V. parahaemolyticus infection.