Publication: Short report: Molecular confirmation of co-infection by pathogenic leptospira spp. and orientia tsutsugamushi in patients with acute febrile illness in Thailand
Issued Date
2013-10-01
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ISSN
00029637
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2-s2.0-84885995205
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.89, No.4 (2013), 797-799
Suggested Citation
Piengchan Sonthayanon, Wirongrong Chierakul, Vanaporn Wuthiekanun, Direk Limmathurotsakul, Premjit Amornchai, Lee D. Smythe, Nicholas P. Day, Sharon J. Peacock Short report: Molecular confirmation of co-infection by pathogenic leptospira spp. and orientia tsutsugamushi in patients with acute febrile illness in Thailand. American Journal of Tropical Medicine and Hygiene. Vol.89, No.4 (2013), 797-799. doi:10.4269/ajtmh.13-0402 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31865
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Title
Short report: Molecular confirmation of co-infection by pathogenic leptospira spp. and orientia tsutsugamushi in patients with acute febrile illness in Thailand
Abstract
Leptospirosis and scrub typhus are major causes of acute febrile illness in rural Asia, where co-infection is reported to occur based on serologic evidence. We re-examined whether co-infection occurs by using a molecular approach. A duplex real-time polymerase chain reaction was developed that targeted a specific 16S ribosomal RNA gene of pathogenic Leptospira spp. and Orientia tsutsugamushi. Of 82 patients with an acute febrile illness who had dual infection on the basis of serologic tests, 5 (6%) had polymerase chain reaction results positive for both pathogens. We conclude that dual infection occurs, but that serologic tests may overestimate the frequency of co-infections. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.