Publication: An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody
Issued Date
2019-01-01
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ISSN
10990801
02693879
02693879
DOI
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2-s2.0-85076762583
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Mahidol University
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SCOPUS
Bibliographic Citation
Biomedical Chromatography. (2019)
Suggested Citation
Tharita Kitisripanya, Witcha Sermpradit, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody. Biomedical Chromatography. (2019). doi:10.1002/bmc.4718 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50365
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Title
An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody
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Abstract
© 2019 John Wiley & Sons, Ltd. There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold–monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5–200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme-linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.