Publication: One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae
Issued Date
2009-06-01
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ISSN
01677012
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2-s2.0-67349175771
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Microbiological Methods. Vol.77, No.3 (2009), 323-325
Suggested Citation
Ratana Lawung, Rungrot Cherdtrakulkiat, Angkana Charoenwatanachokchai, Sunanta Nabu, Wanvisa Suksaluk, Virapong Prachayasittikul One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae. Journal of Microbiological Methods. Vol.77, No.3 (2009), 323-325. doi:10.1016/j.mimet.2009.03.009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27209
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Title
One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae
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Abstract
One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction. © 2009 Elsevier B.V. All rights reserved.