Publication: A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection
Issued Date
2001-01-01
Resource Type
ISSN
00359203
Other identifier(s)
2-s2.0-0035348067
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.95, No.3 (2001), 280-284
Suggested Citation
N. Rahmah, B. H. Lim, A. Khairul Anuar, R. K. Shenoy, V. Kumaraswami, S. Lokman Hakim, P. Chotechuang, K. Kanjanopas, C. P. Ramachandran A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.95, No.3 (2001), 280-284. doi:10.1016/S0035-9203(01)90234-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/26598
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Title
A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection
Abstract
An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0·300, the IgG4 ELISA demonstrated specificity rates of 95·6-100%, sensitivity rates of 96-100%, positive predictive values of 75-100% and negative predictive values of 98·9-100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.