Publication:
Axenic cultivation of Entamoeba histolytica from liver abscess and its zymodeme.

dc.contributor.authorN. Thammapalerden_US
dc.contributor.authorS. Tharavanijen_US
dc.contributor.authorY. Wattanagoonen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-08-10T08:57:56Z
dc.date.available2018-08-10T08:57:56Z
dc.date.issued1993-09-01en_US
dc.description.abstractA local strain of Entamoeba histolytica, the HTH-56: MUTM from a human liver abscess was successfully axenized. The culture was initially established monoxenically in Diamond's TYI-S-33 medium in the presence of Crithidia luciliae and maintained at 34 +/- 0.5 degrees C. After 5 passages it was adapted to axenic cultivation by addition of 0.02% Bacto agar in Diamond's TYI-S-33 medium in place of Crithidia. Subcultures or replacement with fresh complete media were done twice or thrice for 7 days, after which the agar was omitted and a stable culture was obtained. Isoenzyme analysis showed that this strain of E. histolytica belonged to the zymodeme II pattern, which is one out of 10 pathogenic zymodemes of E. histolytica most commonly found among the virulent strains.en_US
dc.identifier.citationThe Southeast Asian journal of tropical medicine and public health. Vol.24, No.3 (1993), 480-483en_US
dc.identifier.issn01251562en_US
dc.identifier.other2-s2.0-0027658181en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/22702
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0027658181&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleAxenic cultivation of Entamoeba histolytica from liver abscess and its zymodeme.en_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0027658181&origin=inwarden_US

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