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Surface modification of silicon dioxide, silicon nitride and titanium oxynitride for lactate dehydrogenase immobilization

dc.contributor.authorPawasuth Saengdeeen_US
dc.contributor.authorWoraphan Chaisriratanakulen_US
dc.contributor.authorWin Bunjongpruen_US
dc.contributor.authorWitsaroot Sripumkhaien_US
dc.contributor.authorAwirut Srisuwanen_US
dc.contributor.authorWutthinan Jeamsaksirien_US
dc.contributor.authorCharndet Hruanunen_US
dc.contributor.authorAmporn Poyaien_US
dc.contributor.authorChamras Promptmasen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThai Microelectronics Center (TMEC)en_US
dc.date.accessioned2018-11-23T09:43:20Z
dc.date.available2018-11-23T09:43:20Z
dc.date.issued2015-05-05en_US
dc.description.abstract© 2014 Elsevier B.V. Three different types of surface, silicon dioxide (SiO2), silicon nitride (Si3N4), and titanium oxynitride (TiON) were modified for lactate dehydrogenase (LDH) immobilization using (3-aminopropyl)triethoxysilane (APTES) to obtain an amino layer on each surface. The APTES modified surfaces can directly react with LDH via physical attachment. LDH can be chemically immobilized on those surfaces after incorporation with glutaraldehyde (GA) to obtain aldehyde layers of APTES-GA modified surfaces. The wetting properties, chemical bonding composition, and morphology of the modified surface were determined by contact angle (CA) measurement, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM), respectively. In this experiment, the immobilized protein content and LDH activity on each modified surface was used as an indicator of surface modification achievement. The results revealed that both the APTES and APTES-GA treatments successfully link the LDH molecule to those surfaces while retaining its activity. All types of tested surfaces modified with APTES-GA gave better LDH immobilizing efficiency than APTES, especially the SiO2surface. In addition, the SiO2surface offered the highest LDH immobilization among tested surfaces, with both APTES and APTES-GA modification. However, TiON and Si3N4surfaces could be used as alternative candidate materials in the preparation of ion-sensitive field-effect transistor (ISFET) based biosensors, including lactate sensors using immobilized LDH on the ISFET surface.en_US
dc.identifier.citationBiosensors and Bioelectronics. Vol.67, (2015), 134-138en_US
dc.identifier.doi10.1016/j.bios.2014.07.057en_US
dc.identifier.issn18734235en_US
dc.identifier.issn09565663en_US
dc.identifier.other2-s2.0-84922336417en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/35458
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84922336417&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemistryen_US
dc.subjectEngineeringen_US
dc.titleSurface modification of silicon dioxide, silicon nitride and titanium oxynitride for lactate dehydrogenase immobilizationen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84922336417&origin=inwarden_US
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