Publication: In vitro study of the effects of plaunotol on oral cell proliferation and wound healing
Issued Date
2011-02-01
Resource Type
ISSN
14772213
10286020
10286020
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2-s2.0-79551605075
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Asian Natural Products Research. Vol.13, No.2 (2011), 149-159
Suggested Citation
Siribang On Piboonniyom Khovidhunkit, Nadiya Yingsaman, Kongtawat Chairachvit, Rudee Surarit, Pornpoj Fuangtharnthip, Amorn Petsom In vitro study of the effects of plaunotol on oral cell proliferation and wound healing. Journal of Asian Natural Products Research. Vol.13, No.2 (2011), 149-159. doi:10.1080/10286020.2010.546790 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11599
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Title
In vitro study of the effects of plaunotol on oral cell proliferation and wound healing
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Abstract
Plaunotol is an acyclic diterpene alcohol extracted from a medicinal plant called plau-noi, Croton stellatopilosus Ohba, and has been widely used for the treatment of gastric ulcers in Japan. The aim of this study was to examine the effects of plaunotol on human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs). To assess the cytotoxic effect, HGFs and HOKs were treated with plaunotol. Subsequently, the morphology of cells was recorded and cells were subjected to MTT assay. To investigate cell proliferation effect, cells were treated with plaunotol and counted with a haemocytometer. To determine wound healing effect, the number of cells repopulated into the wounded areas in monolayer culture and in fibroblast-populated collagen lattice (FPCL) was measured. The results showed that 10 and 1g/ml (33 and 3.3mol/l) plaunotol induced toxicity in HGFs and HOKs, respectively. However, 0.1g/ml (0.33mol/l) plaunotol promoted HGF proliferation and wound healing in monolayer and FPCL models. In contrast, 0.1g/ml plaunotol could not induce HOK proliferation nor in vitro wound healing using monolayer culture, but it induced wound healing in a modified FPCL model. Our data suggested that plaunotol could promote oral cell proliferation and wound healing in vitro and may have an implication on oral wound healing. © 2011 Taylor & Francis.