Publication: β-Thalassaemia/haemoglobin E tissue ferritins - II: A comparison of heart and pancreas ferritins with those of liver and spleen
Issued Date
1990-09-01
Resource Type
ISSN
15728773
09335854
09335854
Other identifier(s)
2-s2.0-0025579584
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biology of Metals. Vol.3, No.3-4 (1990), 227-231
Suggested Citation
Kim Chi Tran, John Webb, David J. Macey, Pensri Pootrakul β-Thalassaemia/haemoglobin E tissue ferritins - II: A comparison of heart and pancreas ferritins with those of liver and spleen. Biology of Metals. Vol.3, No.3-4 (1990), 227-231. doi:10.1007/BF01140584 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15896
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
β-Thalassaemia/haemoglobin E tissue ferritins - II: A comparison of heart and pancreas ferritins with those of liver and spleen
Author(s)
Other Contributor(s)
Abstract
Tissue ferritins from β-thalassaemia/haemoglobin E heart and pancreas were characterized by native PAGE, SDS/PAGE and isoelectric focussing, and compared with those isolated from corresponding liver and spleen tissue. On PAGE; all ferritins consisted of a single band assigned to the protein monomer. Small differences in electrophoretic mobility were found between the bands. The ferritins were resolved by SDS/PAGE into two major subunits, H and L, corresponding to molecular masses of 22.5 kDa and 19 kDa, respectively. The L subunit was predominant in all cases. The isoferritin profiles of all tissue ferritins were remarkably similar; consisting of a complex pattern of bands which were appreciably more basic than those obtained for horse spleen ferritin. The subunit composition and isoferritin profiles of the four tissue ferritins almost certainly reflect the defense mechanism of the body in synthesizing in all four tissue types a more stable long-term iron-storage isoferritin in order to detoxify and store the excess iron present due to the pathological condition of β-thalassaemia/HbE. © 1990 Springer-Verlag.