Publication: Development of cultivation technique for pure Plasmodium falciparum gametocytes.
Issued Date
1995-12-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0029421362
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Mahidol University
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SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health. Vol.26, No.4 (1995), 606-610
Suggested Citation
P. Petmitr, G. Pongvilairat, P. Wilairat Development of cultivation technique for pure Plasmodium falciparum gametocytes.. The Southeast Asian journal of tropical medicine and public health. Vol.26, No.4 (1995), 606-610. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17351
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Title
Development of cultivation technique for pure Plasmodium falciparum gametocytes.
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Abstract
Pure gametocyte culture of Plasmodium falciparum, isolate KT3, from Kanchanaburi Province, Thailand, was successfully established by 5% sorbitol treatment on days 9, 10 and 11 following initiation of culture. Using medium supplemented with 15% human plasma and activated erythrocytes, daily medium change was not required during the cultivation. There was 99% reduction in the numbers of asexual parasites in the culture but the numbers of gametocytes were not affected. Furthermore, the gametocytes could undergo their usual morphological development with retention of function as demonstrated by the appearance of exflagellating microgametocytes, macrogametocytes and of oocyst formation in midgut of infected mosquito.