Publication:
Controlled human malaria infection with a clone of Plasmodium vivax with highquality genome assembly

4

Issued Date

2021-12-08

Resource Type

Article

ISSN

23793708

DOI

10.1172/jci.insight.152465

Other identifier(s)

2-s2.0-85120879529

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

JCI Insight. Vol.6, No.23 (2021)

Suggested Citation

Angela M. Minassian, Yrene Themistocleous, Sarah E. Silk, Jordan R. Barrett, Alison Kemp, Doris Quinkert, Carolyn M. Nielsen, Nick J. Edwards, Thomas A. Rawlinson, Fernando Ramos Lopez, Wanlapa Roobsoong, Katherine J.D. Ellis, Jee Sun Cho, Eerik Aunin, Thomas D. Otto, Adam J. Reid, Florian A. Bach, Geneviève M.C. Labbé, Ian D. Poulton, Arianna Marini, Marija Zaric, Margaux Mulatier, Raquel Lopej Ramon, Megan Baker, Celia H. Mitton, Jason C. Sousa, Nattawan Rachaphaew, Chalermpon Kumpitak, Nongnuj Maneechai, Chayanut Suansomjit, Tianrat Piteekan, Mimi M. Hou, Baktash Khozoee, Kirsty McHugh, David J. Roberts, Alison M. Lawrie, Andrew M. Blagborough, Fay L. Nugent, Iona J. Taylor, Kimberly J. Johnson, Philip J. Spence, Jetsumon Sattabongkot, Sumi Biswas, Julian C. Rayner, Simon J. Draper Controlled human malaria infection with a clone of Plasmodium vivax with highquality genome assembly. JCI Insight. Vol.6, No.23 (2021). doi:10.1172/jci.insight.152465 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/77407

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Title

Controlled human malaria infection with a clone of Plasmodium vivax with highquality genome assembly

Author(s)

Angela M. Minassian
 Yrene Themistocleous
 Sarah E. Silk
 Jordan R. Barrett
 Alison Kemp
 Doris Quinkert
 Carolyn M. Nielsen
 Nick J. Edwards
 Thomas A. Rawlinson
 Fernando Ramos Lopez
 Wanlapa Roobsoong
 Katherine J.D. Ellis
 Jee Sun Cho
 Eerik Aunin
 Thomas D. Otto
 Adam J. Reid
 Florian A. Bach
 Geneviève M.C. Labbé
 Ian D. Poulton
 Arianna Marini
 Marija Zaric
 Margaux Mulatier
 Raquel Lopej Ramon
 Megan Baker
 Celia H. Mitton
 Jason C. Sousa
 Nattawan Rachaphaew
 Chalermpon Kumpitak
 Nongnuj Maneechai
 Chayanut Suansomjit
 Tianrat Piteekan
 Mimi M. Hou
 Baktash Khozoee
 Kirsty McHugh
 David J. Roberts
 Alison M. Lawrie
 Andrew M. Blagborough
 Fay L. Nugent
 Iona J. Taylor
 Kimberly J. Johnson
 Philip J. Spence
 Jetsumon Sattabongkot
 Sumi Biswas
 Julian C. Rayner
 Simon J. Draper

Other Contributor(s)

Faculty of Tropical Medicine, Mahidol University
 Cambridge Institute for Medical Research
 University of Cambridge
 The University of Edinburgh
 University of Oxford
 Walter Reed Army Institute of Research
 Wellcome Sanger Institute
 University of Oxford Medical Sciences Division

Abstract

Controlled human malaria infection (CHMI) provides a highly informative means to investigate host-pathogen interactions and enable in vivo proof-of-concept efficacy testing of new drugs and vaccines. However, unlike Plasmodium falciparum, well-characterized P. vivax parasites that are safe and suitable for use in modern CHMI models are limited. Here, 2 healthy malaria-naive United Kingdom adults with universal donor blood group were safely infected with a clone of P. vivax from Thailand by mosquito-bite CHMI. Parasitemia developed in both volunteers, and prior to treatment, each volunteer donated blood to produce a cryopreserved stabilate of infected RBCs. Following stringent safety screening, the parasite stabilate from one of these donors (PvW1) was thawed and used to inoculate 6 healthy malaria-naive United Kingdom adults by blood-stage CHMI, at 3 different dilutions. Parasitemia developed in all volunteers, who were then successfully drug treated. PvW1 parasite DNA was isolated and sequenced to produce a high-quality genome assembly by using a hybrid assembly method. We analyzed leading vaccine candidate antigens and multigene families, including the vivax interspersed repeat (VIR) genes, of which we identified 1145 in the PvW1 genome. Our genomic analysis will guide future assessment of candidate vaccines and drugs, as well as experimental medicine studies.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/77407

Collections

Scopus 2021