Publication: Engineered Escherichia coli for short-chain-length medium-chain-length polyhydroxyalkanoate copolymer biosynthesis from glycerol and dodecanoate
Issued Date
2013-07-19
Resource Type
ISSN
13476947
09168451
09168451
Other identifier(s)
2-s2.0-84880174964
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.77, No.6 (2013), 1262-1268
Suggested Citation
Chitwadee Phithakrotchanakoon, Verawat Champreda, Sei Ichi Aiba, Kusol Pootanakit, Sutipa Tanapongpipat Engineered Escherichia coli for short-chain-length medium-chain-length polyhydroxyalkanoate copolymer biosynthesis from glycerol and dodecanoate. Bioscience, Biotechnology and Biochemistry. Vol.77, No.6 (2013), 1262-1268. doi:10.1271/bbb.130073 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31278
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Title
Engineered Escherichia coli for short-chain-length medium-chain-length polyhydroxyalkanoate copolymer biosynthesis from glycerol and dodecanoate
Abstract
Short-chain-length medium-chain-length polyhydroxyalkanoate (SCL-MCL PHA) copolymers are promising as bio-plastics with properties ranging from thermoplastics to elastomers. In this study, the hybrid pathway for the biosynthesis of SCL-MCL PHA copolymers was established in recombinant Escherichia coli by coexpression of β-ketothiolase (PhaARe) and NADPH-dependent acetoacetyl-CoA reductase (PhaBRe) from Ralstonia eutropha together with PHA synthases from R. eutropha (PhaCRe), Aeromonas hydrophila (PhaCAh), and Pseudomonas putida (PhaC2Pp) and with (R)-specific enoyl-CoA hydratases from P. putida (PhaJ1Ppand PhaJ4Pp), and A. hydrophila (PhaJAh). When glycerol supplemented with dodecanoate was used as primary carbon source, E. coli harboring various combinations of PhaABCJ produced SCL-MCL PHA copolymers of various monomer compositions varying from C4to C10. In addition, polymer property analysis suggested that the copolymers produced from this recombinant source have thermal properties (lower glass transition and melting temperatures) superior to polyhydroxybutyrate homopolymer.