Publication: Comprehensive Molecular Analysis of Serologically D-Negative and Weak/Partial D Phenotype in Thai Blood Donors
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Issued Date
2019-01-01
Resource Type
ISSN
16603818
16603796
16603796
Other identifier(s)
2-s2.0-85063981145
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Mahidol University
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SCOPUS
Bibliographic Citation
Transfusion Medicine and Hemotherapy. (2019)
Suggested Citation
Jairak Thongbut, Loann Raud, Claude Férec, Charuporn Promwong, Pornlada Nuchnoi, Yann Fichou Comprehensive Molecular Analysis of Serologically D-Negative and Weak/Partial D Phenotype in Thai Blood Donors. Transfusion Medicine and Hemotherapy. (2019). doi:10.1159/000499087 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/52371
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Title
Comprehensive Molecular Analysis of Serologically D-Negative and Weak/Partial D Phenotype in Thai Blood Donors
Abstract
© 2019 S. Karger AG, Basel. Background: Molecular genetics of the Rh system has been extensively studied in Caucasians, Black Africans, East Asians, and Indians more recently. In this work, we sought to investigate the molecular basis of variant D expression in the Thai population, which remains unknown. Materials and Methods: Blood samples from 450 Thai donors showing the variant D phenotype were collected. The RHD gene was analyzed by quantitative multiplex polymerase chain reaction of short fluorescent fragments and/or Sanger sequencing. Results: The most frequent alleles in 200 D-negative and 121 DEL samples were the whole RHD gene deletion and the Asian DEL alleles, respectively. In 129 weak/partial D samples, 36 variant alleles were identified, including eight novel alleles. RHD∗06.03, which is common in variant D samples from South China, is the most prevalent variant allele, followed by the recently reported Indian RHD∗01W.150 allele. Discussion: For the first time, a comprehensive overview of the nature and distribution of variant RHD alleles in Thailand is reported. It is a milestone to pave the way towards improvement of the current screening strategy to identify DEL donors accurately. The next step will be the design and implementation of a simple molecular test for screening the most frequent alleles, specifically in this population.