Publication: Scanning electron microscope characterization of abrasion in human teeth.
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Accepted Date
2014-07-22
Issued Date
2014-09
Resource Type
Language
eng
ISSN
0125-5614 (printed)
Rights
Mahidol University
Rights Holder(s)
Faculty of Dentistry Mahidol University
Bibliographic Citation
Worawongvasu R. Scanning electron microscope characterization of abrasion in human teeth. M Dent J. 2014; 34(3): 279-88.
Suggested Citation
Ratthapong Worawongvasu, รัฐพงษ์ วรวงศ์วสุ Scanning electron microscope characterization of abrasion in human teeth.. Worawongvasu R. Scanning electron microscope characterization of abrasion in human teeth. M Dent J. 2014; 34(3): 279-88.. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/1127
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Title
Scanning electron microscope characterization of abrasion in human teeth.
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Corresponding Author(s)
Abstract
Objectives: The purpose of this study was to examine the surface changes of
abrasion in human teeth by scanning electron microscopy.
Materials and methods: Ten human premolars with cervical abrasion from 10
patients requiring tooth extraction, one tooth from each patient, were used in
this study. The specimens were collected from the Department of Oral and
Maxillofacial Surgery, Faculty of Dentistry, Mahidol University and private
dental clinics. After extractions, all teeth were stored in 10% formalin until
required. The specimens were cut in mesiodistal directions, immersed in
5.25% sodium hypochlorite and then dehydrated using increasing ethanol
concentrations (two changes 15 minutes each): 50%, 60%, 70%, 85%, 95%,
100% and dried by leaving the specimens at room temperature for 24 hours,
mounted on aluminum stubs, coated with gold, 100-300 Å thick, with an ion
sputter coater, and viewed with a JEOL JSM-6610 LV scanning electron
microscope, at seven magnifications: X20, X30, X100, X500, X1,000, X3,500,
and X10,000. The photomicrographs were described.
Results: In the abraded areas which were limited to the enamel surfaces,
most of the enamel surfaces appeared uniformly smooth and showed
presence of horizontal, vertical, and oblique scratches. In the abraded areas
which were deep and extended into the dentin, dentinal tubules were
exposed and could be observed in oblique and longitudinal sections. In some
areas, the enamel surfaces showed dissolution of enamel rod ends which
gave the enamel surface a honeycomb appearance. In other areas, the dentin
surfaces appeared rough with numerous openings of dentinal tubules. Parallel
linear scratches arranged in various directions were also noted. Dentinal
tubules showed a variation in the diameter of the openings, from patent to
completely obliterated. In a few areas, an opening of the dentinal tubule was
surrounded by a dentin matrix which consisted of a network of collagen fibers.
In one tooth, two vertical fissures were seen in the abraded area.
Conclusions: The abraded enamel surfaces appear smooth with a variable
number of parallel scratch marks which are arranged in various directions. The
abraded dentin surfaces show linear scratches similar to those of the abraded
enamel and contain numerous openings of dentinal tubules. Evidence of
erosion and abfraction is found.