Publication: Characterization and localization of saposin-like protein-2 (SAP-2) in Fasciola gigantica
Issued Date
2011-06-01
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ISSN
14321955
09320113
09320113
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2-s2.0-79959372933
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology Research. Vol.108, No.6 (2011), 1493-1500
Suggested Citation
Pornanan Kueakhai, Krai Meemon, Narin Changklungmoa, Kulathida Chaithirayanon, Suda Riengrojpitak, Prasert Sobhon Characterization and localization of saposin-like protein-2 (SAP-2) in Fasciola gigantica. Parasitology Research. Vol.108, No.6 (2011), 1493-1500. doi:10.1007/s00436-010-2201-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12035
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Title
Characterization and localization of saposin-like protein-2 (SAP-2) in Fasciola gigantica
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Abstract
Fasciola gigantica saposin-like protein-2 (FgSAP-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. In this study, we have cloned and expressed FgSAP-2 and produced the antibody against this recombinant protein. Rabbit antiserum against rFgSAP-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. In situ hybridization and immunofluorescence detection revealed the presence of SAP-2 mRNA transcripts and proteins in the cecal epithelial cells of 4-week-old juvenile and adult parasites, but not in the metacercariae and newly excysted juveniles. Moreover, SAP-2 is present only in the cecal epithelial cells lining the distal part of the digestive tract, but not in the tegumental-type epithelium lining the proximal part of the digestive tract. The rFgSAP-2 reacted with antisera from rabbits infected with F. gigantica metacercariae collected at 5 weeks, but not at 2 weeks after infection. Anti-rFgSAP-2 did not exhibit any cross-reactivity with the other parasites' antigens, including Opisthorchis viverrini, Eurytrema pancreaticum, Cotylophoron cotylophorum, Fischoederius cobboldi, Gigantocotyle explanatum, Paramphistomum cervi, Setaria labiato-papillosa, and Haemonchus placei. This finding indicated that SAP-2 is a unique protein that is expressed only in late juvenile and adult F. gigantica, and it could be considered for immunodiagnostic and as a vaccine candidate for fasciolosis. © 2010 Springer-Verlag.