Publication: De novo and salvage biosynthesis of pteroylpentaglutamates in the human malaria parasite, Plasmodium falciparum
Issued Date
1989-01-01
Resource Type
ISSN
01666851
Other identifier(s)
2-s2.0-0024465225
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Biochemical Parasitology. Vol.32, No.1 (1989), 25-37
Suggested Citation
Jerapan Krungkrai, H. Kyle Webster, Yongyuth Yuthavong De novo and salvage biosynthesis of pteroylpentaglutamates in the human malaria parasite, Plasmodium falciparum. Molecular and Biochemical Parasitology. Vol.32, No.1 (1989), 25-37. doi:10.1016/0166-6851(89)90126-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15724
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Title
De novo and salvage biosynthesis of pteroylpentaglutamates in the human malaria parasite, Plasmodium falciparum
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Abstract
Plasmodium falciparum was shown to synthesize pteroylpolyglutamate de novo from guanosine 5′-triphosphate (GTP), p-aminobenzoate (PABA), and l-glutamate (l-Glu). The parasite also had the capacity to synthesize pteroypolyglutamate from both intact and degradation moieties (p-aminobenzoylglutamate and pterin-aldehyde) of exogenous folate added into the growth medium. The major product was identified as 5-methyl-tetrahydroteroylpentaglutamate following exposure to pteroylpolyglutamate hydrolase and oxidative degradation of the C9-N10 bond in the molecule and identification of products by reversed-phase high performance liquid chromatography. Inhibition of pteroylpentaglutamate synthesis from the radiolabelled metabolic precursors (GTP, PABA, l-Glu) and folate by the antifolate antimalarials, pyrimethamine and sulfadoxine at therapeutic concentrations, may suggest the existence of a unique biosynthetic pathway in the malaria parasite. © 1989.