Publication: Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay
1
Issued Date
2005-06-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-22244475520
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.72, No.6 (2005), 694-701
Suggested Citation
Warunee Ngrenngarmlert, Jesse J. Kwiek, Deborah D. Kamwendo, Kimberly Ritola, Ronald Swanstrom, Chansuda Wongsrichanalai, R. Scott Miller, Wanida Ittarat, Steven R. Meshnick Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay. American Journal of Tropical Medicine and Hygiene. Vol.72, No.6 (2005), 694-701. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16581
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Title
Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay
Abstract
We developed a novel Plasmodium falciparum genotyping strategy based on the heteroduplex tracking assay (HTA) method commonly used to genotype viruses. Because it can detect both sequence and size polymorphisms, we hypothesized that HTA is more sensitive than current methods. To test this hypothesis, we compared the ability of HTA and a nested polymerase chain reaction (PCR) to detect genetic diversity in 17 Thai samples. The HTA detected more MSP1 sequence variants in eight isolates (47%), less sequence variants in three isolates (18%), and an equal number of sequence variants in six isolates (35%), suggesting that HTA is equal to or more sensitive than the nested PCR. This study is a proof of concept that HTA is a sensitive allelic discrimination method able to determine genetic diversity in P. falciparum and warrants its use in studies of antimalarial drug efficacy. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.