Publication: The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat
Issued Date
2013-09-01
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ISSN
1432072X
03028933
03028933
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2-s2.0-84884281807
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Microbiology. Vol.195, No.9 (2013), 671-674
Suggested Citation
Panithi Leesukon, Wilaiwan Wirathorn, Tatsanee Chuchue, Nisanart Charoenlap, Skorn Mongkolsuk The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat. Archives of Microbiology. Vol.195, No.9 (2013), 671-674. doi:10.1007/s00203-013-0913-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31229
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Title
The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat
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Abstract
The presence of the widely used selectable antibiotic marker, tetA(C), unexpectedly increased the sensitivity of Pseudomonas aeruginosa PAO1 to the superoxide-generating herbicide, paraquat. A DNA fragment spanning the first 99 amino acids of TetA(C) was sufficient to confer paraquat sensitivity. The TetA(C)-induced paraquat sensitive phenotype was observed in other Gram-negative bacteria such as Agrobacterium tumefaciens, Salmonella enterica ser. Typhimurium and Xanthomonas campestris suggesting that this is a general property of tetA(C). This finding serves as a cautionary note for those using tetA(C) as a selectable marker for genetic manipulations in studies using paraquat either as a superoxide stress generator or a redox cycling drug. © 2013 Springer-Verlag Berlin Heidelberg.