Publication: Production of viral antigens in culture fluid of C6/36 mosquito cell line infected with dengue type 4 virus strains isolated from patients with different clinical severities
Issued Date
1999-08-01
Resource Type
ISSN
0001723X
Other identifier(s)
2-s2.0-0032873277
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Virologica. Vol.43, No.4 (1999), 213-218
Suggested Citation
C. Chanyasanha, F. Hasebe, R. Matias, A. Igarashi Production of viral antigens in culture fluid of C6/36 mosquito cell line infected with dengue type 4 virus strains isolated from patients with different clinical severities. Acta Virologica. Vol.43, No.4 (1999), 213-218. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25436
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Production of viral antigens in culture fluid of C6/36 mosquito cell line infected with dengue type 4 virus strains isolated from patients with different clinical severities
Author(s)
Other Contributor(s)
Abstract
Viral antigen production was examined in the culture fluid of Aedes albopictus clone C6/36 cell line incubated at 28°C and 37°C after infection with four strains of dengue type 4 (DEN-4) virus which were isolated from patients with different clinical severities. During the observation period from day 1 to day 18, the number of infected cells at each day for all four strains did not show any significant difference (P >0.05). Antigen production as determined by the hemagglutination (HA) test and sandwich enzyme linked immunosorbent assay (ELISA) was higher at 28°C than at 37°C for all four DEN-4 virus strains examined. The amount of viral antigen produced was highest for CT93-74 strain (dengue hemorrhagic fever syndrome (DHF) grade II) and was significantly different in comparison to other strains (P <0.001). This strain was followed by CT93-158 and CT93-129 strains (both of DHF grade I), and CT93-77 strain (dengue fever (DF)). The viral antigen production was apparently proportional to the clinical severity of the patient from whom the virus was isolated. These results show that CT93-74 strain could be used to produce DEN-4 virus antigen of sufficiently high titer in the C6/36 cell culture instead of classical extraction of this antigen from suckling mouse brains.