Publication:
Single nucleoprotein residue determines influenza A virus sensitivity to an intertypic suppression mechanism

dc.contributor.authorJaraspim Narkpuken_US
dc.contributor.authorSamaporn Teeravechyanen_US
dc.contributor.authorPilaipan Puthavathanaen_US
dc.contributor.authorAnan Jongkaewwattanaen_US
dc.contributor.authorPeera Jaru-Ampornpanen_US
dc.contributor.otherThailand National Science and Technology Development Agencyen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-21T07:57:28Z
dc.date.accessioned2019-03-14T08:03:48Z
dc.date.available2018-12-21T07:57:28Z
dc.date.available2019-03-14T08:03:48Z
dc.date.issued2017-06-01en_US
dc.description.abstract© 2017 Elsevier Inc. Several mechanisms underlying intertypic interference between co-infecting influenza types A and B viruses (IAV and IBV) have been proposed. We have recently described one in which IBV's nucleoprotein (BNP) sequestered IAV's nucleoprotein (ANP) and suppressed IAV polymerase and growth. However, its anti-IAV capacity and limitations have not been fully explored. Here, we showed that BNP's inhibitory effect was more potent toward a wide array of avian IAVs, whereas human IAVs revealed moderate resistance. BNP sensitivity was largely determined by ANP's residue 343 at the NP oligomerization interface. An avian IAV polymerase carrying an NP-V343L mutation switched from being highly BNP-sensitive to moderately BNP-resistant, and vice versa for a human IAV polymerase carrying a reverse mutation. To highlight its capacity, we demonstrated that the polymerases of highly-pathogenic H5N1 and the pandemic 2009 (H1N1) strains are strongly inhibited by BNP. Our work provides insights into lineage-specific sensitivity to BNP-mediated intertypic interference.en_US
dc.identifier.citationVirology. Vol.506, (2017), 99-109en_US
dc.identifier.doi10.1016/j.virol.2017.03.015en_US
dc.identifier.issn10960341en_US
dc.identifier.issn00426822en_US
dc.identifier.other2-s2.0-85016408500en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/42781
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85016408500&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleSingle nucleoprotein residue determines influenza A virus sensitivity to an intertypic suppression mechanismen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85016408500&origin=inwarden_US

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