Publication: Antioxidant, a-Glucosidases and a-Amylase Inhibitory Activities of Persicaria odorata
Issued Date
2021-07-01
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ISSN
16851994
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2-s2.0-85108801623
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Mahidol University
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SCOPUS
Bibliographic Citation
Chiang Mai University Journal of Natural Sciences. Vol.20, No.3 (2021), 1-14
Suggested Citation
Kanya Thongra-ar, Piyanuch Rojsanga, Savita Chewchinda, Supachoke Mangmool, Pongtip Sithisarn Antioxidant, a-Glucosidases and a-Amylase Inhibitory Activities of Persicaria odorata. Chiang Mai University Journal of Natural Sciences. Vol.20, No.3 (2021), 1-14. doi:10.12982/CMUJNS.2021.051 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/79343
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Title
Antioxidant, a-Glucosidases and a-Amylase Inhibitory Activities of Persicaria odorata
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Abstract
The objects of this study were to determine the effects to reactive oxygen species and antioxidant enzymes levels in HEK-293 cells and inhibition of α-glucosidases and α-amylase enzymes of extracts from Persicaria odorata or phak phaeo. The ethanol extracts from the leaves and the stems of phak phaeo were investigated for their 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging activities (IC50 were 7.74 ± 0.47 and 7.91 ± 0.43 µg/mL, respectively). Cellular antioxidant effects in human embryonic kidney-293 (HEK-293) cells with these extracts (0.1 mg/mL) also increased the mRNA expressions of manganese superoxide dismutase (Mn-SOD), glutathione peroxidase 1 (GPx-1), catalase and glutathione reductase (GRe). The leaf extract showed the higher efficacies in the induction of the mRNA expressions of Mn-SOD, GPx-1 and GRe while the stem extract exhibited a stronger effect to the induction of catalase. Phak phaeo in vitro inhibitory effects to α-glucosidase enzyme (IC50 values of 9.82 ± 1.64 and 13.99 ± 1.45 µg/mL, respectively and also strong inhibition to a-amylase with IC50 values of 90.66 ± 8.75 and 19.96 ± 5.37 µg/mL, respectively). Lineweaver-Burk plot demonstrated that phak phaeo extracts inhibited a-glucosidase and a-amylase in non-competitive manners. Total phenolic and total flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride methods (the leaf and stem extracts were 22.89 ± 9.16 and 22.27 ± 8.77 g gallic acid equivalent in 100 g extract (g% GAE) and 7.20 ± 3.61 and 4.06 ± 1.73 g quercetin equivalent in 100 g extract (g% QE), respectively).