Publication: Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.
Issued Date
2016-01
Resource Type
Language
eng
ISSN
1305-7456 (printed)
1305-7464 (electronic)
1305-7464 (electronic)
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Mahidol University
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PMC
Bibliographic Citation
Vajrabhaya LO, Korsuwannawong S. Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays. Eur J Dent. 2016 Jan-Mar;10(1):134-8.
Suggested Citation
Suwanna Korsuwannawong, สุวรรณา ก่อสุวรรณวงศ์, La‑Ongthong Vajrabhaya, ละอองทอง วัชราภัย Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.. Vajrabhaya LO, Korsuwannawong S. Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays. Eur J Dent. 2016 Jan-Mar;10(1):134-8.. doi:10.4103/1305-7456.175701 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/1023
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Title
Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.
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Abstract
Objectives:
The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red
uptake (NRU) assays of
Clinacanthus nutans
cytotoxicity.
Materials and Methods:
Mouse fibroblast
(L929) cells were exposed
to 0.01%, 0.1%, 0.25%, and 0.5%
(W/V)
C. nutans
in a 96-cluster
-well-culture plate for 24 h. The cell viability after exposure to
C. nutans
was determined by MTT and NRU assays in separate tissue culture plates. The two assays were compared through an
intra‑class correlation coefficient
(ICC) analysis.
Results:
No significant differences in cytotoxicity were noted between the two
assays (
P
> 0.05). The ICC values for agreement between two assays for the negative and positive control groups and
C. nutans
concentrations of 0.01%, 0.1%, 0.25%, and 0.5% were 0.84, 0.83, 0.77, 0.68, 0.74, and 0.71, respectively.
Conclusion:
In general, the
MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of
C. nutans
.