Publication: Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man)
Issued Date
2004-12-22
Resource Type
ISSN
1355557X
Other identifier(s)
2-s2.0-10844279299
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Aquaculture Research. Vol.35, No.15 (2004), 1415-1420
Suggested Citation
Khattiya Akarasanon, Praneet Damrongphol, Wandee Poolsanguan Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research. Vol.35, No.15 (2004), 1415-1420. doi:10.1111/j.1365-2109.2004.01163.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21051
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man)
Other Contributor(s)
Abstract
Long-term cryopreservation of the giant freshwater prawn. Macrobrachium rosenbergii, spermatophores using glycerol (Gly) and ethylene glycol (EG) as cryoprotective agents (CPAs) was studied. The tolerance of sperm to cryopreservation was evaluated on the basis of sperm survival and fertilizing ability. The survival of the sperm was determined by trypan blue staining, while the fertilizing ability was assessed from artificial insemination of the cryopreserved spermatophores. The rates of embryo survival on day 5 after spawning and of spermatophores capable of producing embryos survived to hatching were determined. Storage of spermatophores at - 20°C without CPA for a short period of up of 1-5 days decreased the sperm survival significantly and did not preserve fertilizing ability. Preservation at - 20°C in the presence of 10% or 20% Gly or of 10% or 20% EG offered a simple and efficient short-term storage up to 10 days. For a long-term storage, cryopreservation in the presence of 20% EG at - 196°C was more efficient than at - 20°C. High sperm survival rates and high fertilizing ability were recorded from those cryopreserved at - 196°C for up to 150 days. High sperm survival rates with moderate levels of fertilizing ability were obtained from those cryopreserved at - 20°C for not more than 30 days. The results indicate that preservation at - 196°C with 20% EG is a suitable procedure for long-term storage of the giant freshwater prawn spermatophores.