Publication: Adhesion of Streptococcus mutans on different types of orthodontic elastic O-rings
Issued Date
2014-01-27
Resource Type
ISSN
16627482
16609336
16609336
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2-s2.0-84892685123
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Mahidol University
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SCOPUS
Bibliographic Citation
Applied Mechanics and Materials. Vol.487, (2014), 75-79
Suggested Citation
Yadana Aung, Suwannee Luppanapornlarp, Sroisiri Thaweboon, Supatchai Boonpratham, Surachai Dechkunakorn, Boonyanit Thaweboon Adhesion of Streptococcus mutans on different types of orthodontic elastic O-rings. Applied Mechanics and Materials. Vol.487, (2014), 75-79. doi:10.4028/www.scientific.net/AMM.487.75 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33829
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Title
Adhesion of Streptococcus mutans on different types of orthodontic elastic O-rings
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Abstract
Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic elastic O-rings. Materials and methods: In vitro experiment, the adhesion of S. mutans on 4 types of elastic Orings [3M Unitek (USA), Dynaflex (USA), Thai elastic (Thailand), W&H (China)] were investigated. The saliva-coated samples were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37 ̇C with 10% CO2 for 2 hours at 100 rpm. The adhered bacterial cells were fixed by using 99% methanol, then stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density at 590 nm. Surface area of each sample was calculated by using the formula [surface area= π2 (R2 - r2)] and surface roughness was investigated by scanning electron microscopy. The data were analyzed with Kolmogorov- Smirnov test, Levene test, one way ANOVA and Tukey HSD post hoc test. Results: High bacteria adhesion was found on 3M Unitek, whereas moderate adhesion was noted on Thai elastic and W&H. The lowest adhesion was found on Dynaflex. Conclusion: Surface area and surface roughness were factors that contributed to the amount of S. mutans adhesion on orthodontic elastic O-rings. © (2014) Trans Tech Publications, Switzerland.