Publication: Tiliacora triandra (Colebr.) diels leaf aqueous extract inhibits hepatic glucose production in HepG2 cells and type 2 diabetic rats
Issued Date
2021-01-01
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ISSN
14203049
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2-s2.0-85102678629
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecules. Vol.26, No.5 (2021)
Suggested Citation
Tipthida Pasachan, Acharaporn Duangjai, Atcharaporn Ontawong, Doungporn Amornlerdpison, Metee Jinakote, Manussabhorn Phatsara, Sunhapas Soodvilai, Chutima Srimaroeng Tiliacora triandra (Colebr.) diels leaf aqueous extract inhibits hepatic glucose production in HepG2 cells and type 2 diabetic rats. Molecules. Vol.26, No.5 (2021). doi:10.3390/molecules26051239 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76396
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Title
Tiliacora triandra (Colebr.) diels leaf aqueous extract inhibits hepatic glucose production in HepG2 cells and type 2 diabetic rats
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Abstract
This study investigated the effects of Tiliacora triandra (Colebr.) Diels aqueous extract (TTE) on hepatic glucose production in hepatocellular carcinoma (HepG2) cells and type 2 diabetic (T2DM) conditions. HepG2 cells were pretreated with TTE and its major constituents found in TTE, epicatechin (EC) and quercetin (QC). The hepatic glucose production was determined. The in vitro data were confirmed in T2DM rats, which were supplemented daily with 1000 mg/kg body weight (BW) TTE, 30 mg/kg BW metformin or TTE combined with metformin for 12 weeks. Results demonstrate that TTE induced copper-zinc superoxide dismutase, glutathione peroxidase and catalase genes, similarly to EC and QC. TTE decreased hepatic glucose production by downregulating phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) and increasing protein kinase B and AMP-activated protein kinase phosphorylation in HepG2 cells. These results correlated with the antihyperglycemic, antitriglyceridemic, anti-insulin resistance, and antioxidant activities of TTE in T2DM rats, similar to the metformin and combination treatments. Consistently, impairment of hepatic gluconeogenesis in T2DM rats was restored after single and combined treatments by reducing PEPCK and G6Pase genes. Collectively, TTE could potentially be developed as a nutraceutical product to prevent glucose overproduction in patients with obesity, insulin resistance, and diabetes who are being treated with antidiabetic drugs.