Publication: Quantitative analysis of Hb Bart's in cord blood by capillary electrophoresis system
Issued Date
2011-07-01
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ISSN
14320584
09395555
09395555
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2-s2.0-79960282616
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Mahidol University
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SCOPUS
Bibliographic Citation
Annals of Hematology. Vol.90, No.7 (2011), 741-746
Suggested Citation
Thongperm Munkongdee, Dalad Pichanun, Punnee Butthep, Sumonmaln Klamchuen, Veeradet Chalermpolprapa, Pranee Winichagoon, Saovaros Svasti, Suthat Fucharoen Quantitative analysis of Hb Bart's in cord blood by capillary electrophoresis system. Annals of Hematology. Vol.90, No.7 (2011), 741-746. doi:10.1007/s00277-010-1137-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12439
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Title
Quantitative analysis of Hb Bart's in cord blood by capillary electrophoresis system
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Abstract
It has long been recognized that the presence of hemoglobin (Hb) Bart's in newborn's blood is associated with α-thalassemia. However, the automated high-performance liquid chromatography or low-performance liquid chromatography system is unable to quantify the amount of Hbs Bart's and H, which are eluted at the retention time close to 0 min. This study used automatic capillary electrophoresis (CE) system to diagnose various types of α-thalassemia in 587 cord blood samples, including 429 normal α-globin genotype, 120 cases of thalassemia with one α-globin gene defect, 34 cases with two α-globin genes defect, and four cases with three α-globin genes defect. The result showed that the level of Hb Bart's in cord blood was increased accordingly with the increasing numbers of the defective α-globin genes. In addition, Hb Bart's level at 0.2%, as measured by CE, can be used as a cut-off point for α-thalassemia diagnosis in newborns. © 2010 Springer-Verlag.