Publication: Development of multiplex pyrosequencing for HLA-B*57:01 screening using single nucleotide polymorphism haplotype
Issued Date
2014-01-01
Resource Type
ISSN
13652710
02694727
02694727
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2-s2.0-84906763426
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Pharmacy and Therapeutics. Vol.39, No.5 (2014), 545-550
Suggested Citation
N. Sankuntaw, S. Chantarangsu, W. Chantratita, S. Sungkanuparph, S. Kiertiburanakul, V. Lulitanond Development of multiplex pyrosequencing for HLA-B*57:01 screening using single nucleotide polymorphism haplotype. Journal of Clinical Pharmacy and Therapeutics. Vol.39, No.5 (2014), 545-550. doi:10.1111/jcpt.12175 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34711
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Title
Development of multiplex pyrosequencing for HLA-B*57:01 screening using single nucleotide polymorphism haplotype
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Abstract
What is known and objective Abacavir (ABC) is a commonly used nucleoside reverse-transcriptase inhibitor with potent antiviral activity against HIV-1. The US Food and Drug Administration and international HIV treatment guidelines recommend HLA-B 57:01 screening before initiating treatment with ABC. The current standard method for HLA-B 57:01 screening is limited by its high-cost, time-consuming and labour-intensive procedure with the requirement of a specialized laboratory. Our study aims to develop a more reliable screening test by selecting rs3093726 as an additional single nucleotide polymorphism (SNP) to combine with rs2395029 for multiplex pyrosequencing development. It offers high-accuracy, cost-effective and rapid detection. Methods Multiplex pyrosequencing was developed for HLA-B 57:01 screening using rs2395029 and rs3093726 as a surrogate marker and tested in 130 Thai subjects in parallel with singleplex pyrosequencing of each SNP and the standard sequence-based method. Results and discussion Multiplex pyrosequencing showed 100% concordance when compared with both singleplex pyrosequencing and standard sequence-based method. This method showed 100% of negative predictive value (NPV), positive predictive value (PPV), specificity and sensitivity. What is new and conclusion Multiplex pyrosequencing is a powerful tool for HLA-B 57:01 screening using the rs2395029 and rs3093726 haplotype genotyping as surrogate marker for this HLA-B. The assay provides accurate, cost-effective and rapid detection of this haplotype. It can be applied for ABC hypersensitivity screening of the Thai population before initiating treatment with ABC. © 2014 John Wiley & Sons Ltd.
