Publication: Rapid and simple identification of human pathogenic heterophyid intestinal fluke metacercariae by PCR-RFLP
Issued Date
2011-12-01
Resource Type
ISSN
18730329
13835769
13835769
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2-s2.0-82155166402
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.60, No.4 (2011), 503-506
Suggested Citation
Urusa Thaenkham, Orawan Phuphisut, Wallop Pakdee, Nirundorn Homsuwan, Surapol Sa-nguankiat, Jitra Waikagul, Yukifumi Nawa, Do Trung Dung Rapid and simple identification of human pathogenic heterophyid intestinal fluke metacercariae by PCR-RFLP. Parasitology International. Vol.60, No.4 (2011), 503-506. doi:10.1016/j.parint.2011.09.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11960
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Title
Rapid and simple identification of human pathogenic heterophyid intestinal fluke metacercariae by PCR-RFLP
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Abstract
Six species of heterophyid intestinal flukes (HIFs) constitute the major endemic zoonotic fish-borne pathogens in Asia: Haplorchis taichui, H. pumilio, H. yokogawai, Procerovum varium, Stellantchasmus falcatus, and Centrocestus formosanus. Several different species of these parasites are often found co-infecting the same second intermediate fish host. Because of their morphological similarities, differentiating between species of HIF met acercariae is difficult, time-consuming, and frequently results in misidentification. In this study, we aimed to develop an efficient and accurate method of identifying metacercariae of these 6 HIFs. Metacercariae were roughly grouped together, based on morphological characteristics seen under a stereomicroscope. Then, PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the exact species of each metacercaria, using the 28S ribosomal RNA gene as the genetic marker and MboII as the restriction enzyme. Using a combination of morphological and molecular methods eliminates the need for DNA sequencing and infecting animal subjects to obtain adult worms, increases accuracy, and decreases the need for laborious morphological identification. Because the method is simple, rapid, and relatively cheap compared with PCR-sequencing, it may be an effective tool for epidemiological studies of HIFs in endemic areas. © 2011.