Publication: Hemoglobin Pakse: Presence on red blood cell membrane and detection by polymerase chain reaction - Single-strand conformational polymorphism
Issued Date
2004-08-01
Resource Type
ISSN
09255710
Other identifier(s)
2-s2.0-4444286637
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Hematology. Vol.80, No.2 (2004), 136-139
Suggested Citation
Chairat Turbpaiboon, Atchasai Siritantikorn, Wanna Thongnoppakhun, Duangkamon Bunditworapoom, Chanin Limwongse, Pa Thai Yenchitsomanus, Noppadol Siritanaratkul, Prapon Wilairat Hemoglobin Pakse: Presence on red blood cell membrane and detection by polymerase chain reaction - Single-strand conformational polymorphism. International Journal of Hematology. Vol.80, No.2 (2004), 136-139. doi:10.1532/IJH97.A20402 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21595
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Hemoglobin Pakse: Presence on red blood cell membrane and detection by polymerase chain reaction - Single-strand conformational polymorphism
Other Contributor(s)
Abstract
Nondeletional gene mutations giving rise to α-thalassemia can be found at polymorphic frequency in Southeast Asia. Although the most common is hemoglobin Constant Spring (Hb CS), caused by a termination codon mutation (UAA → CAA, Gln) in the α2-globin gene and resulting in reduced synthesis of the elongated α-globin variant, Hb Pakse (UAA → UAU, Tyr) also has been observed at a significant prevalence. Western blot analysis of ghost membrane proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis from an individual with α-thal 1/Hb Pakse revealed the existence of a higher molecular weight globin of 18 kd consistent with an αPakse-globin chain. The presence of αPakse- globin on membranes of Hb Pakse-containing red blood cells affords an explanation for the severity of anemia observed in such patients. However, because the 2 Hb variants cannot be distinguished by current biochemical techniques, we developed a convenient single-tube polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) protocol for the simultaneous diagnosis of Hb CS and Hb Pakse by amplifying a short fragment covering the termination codon of the α2-globin gene. This PCR-SSCP method required no internal control coamplification or use of restriction enzymes and has the potential of identifying all the other possible termination codon mutations in a single reaction with only 1 pair of primers. ©2004 The Japanese Society of Hematology.