Publication: A haplosporidian parasite associated with high mortality and slow growth in Penaeus (Litopenaeus) vannamei cultured in Indonesia
8
Issued Date
2012-11-05
Resource Type
ISSN
00448486
Other identifier(s)
2-s2.0-84866707645
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Aquaculture. Vol.366-367, (2012), 85-89
Suggested Citation
Heny Budi Utari, Saengchan Senapin, Chatlada Jaengsanong, Timothy W. Flegel, Maleeya Kruatrachue A haplosporidian parasite associated with high mortality and slow growth in Penaeus (Litopenaeus) vannamei cultured in Indonesia. Aquaculture. Vol.366-367, (2012), 85-89. doi:10.1016/j.aquaculture.2012.09.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13373
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A haplosporidian parasite associated with high mortality and slow growth in Penaeus (Litopenaeus) vannamei cultured in Indonesia
Other Contributor(s)
Abstract
High mortality of juvenile Penaeus (Litopenaeus) vannamei less than one month old in Indonesia has been observed in shrimp farms and hatcheries since 2007. The gross signs of disease included shrinkage of the hepatopancreas, flaccid bodies, cuticular melanization and retarded growth. Overall loss in the severely-infected farms was 60 to 90% due to progressive mortality. The loss since 2007 is estimated to be more than 5. million. US. dollars. PCR testing of shrimp samples for major viral pathogens gave negative results, but histopathological analysis of hepatopancreatic (HP) tissue showed evidence of a haplosporidian parasite indicated by cytoplasmic multinucleate plasmodia and trophonts in tubule epithelial cells. Plasmodia caused disruption of HP tubule epithelial cells when they discharged trophonts into the tubular lumen. To confirm the presence of haplosporidia, PCR was performed to amplify a small subunit rRNA gene (SSU rDNA) fragment and a cloned 1.5. kb amplicon showed 96% sequence identity to that of a haplosporidian previously described in P. vannamei from Belize, Central America suggesting that the Indonesian haplosporidian was either conspecific or very closely related. In situ hybridization assays using a probe based on this amplicon gave positive results specifically with the putative haplosporidian lesions. Prevalence of infections in broodstock and ponds from 2004 to 2010 suggested that the haplosporidian disease outbreaks had resulted from stocking of infected post-larvae. © 2012 Elsevier B.V.