Publication: Xanthomonas oryzae pv. oryzae recA is transcribed and regulated from multiple promoters
Issued Date
2001-04-01
Resource Type
ISSN
03781097
Other identifier(s)
2-s2.0-0035313430
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.197, No.1 (2001), 35-40
Suggested Citation
Rojana Sukchawalit, Skorn Mongkolsuk Xanthomonas oryzae pv. oryzae recA is transcribed and regulated from multiple promoters. FEMS Microbiology Letters. Vol.197, No.1 (2001), 35-40. doi:10.1016/S0378-1097(01)00080-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26470
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Xanthomonas oryzae pv. oryzae recA is transcribed and regulated from multiple promoters
Author(s)
Other Contributor(s)
Abstract
Transcription regulation of Xanthomonas oryzae pv. oryzae recA was characterized. Primer extension experiments showed that recA is transcribed from three promoters designated P1, P2 and P3. The sequences of -10 and -35 regions of these promoters have moderate homology to the proposed consensus sequence for a Xanthomonas promoter. Putative SOS boxes were identified in the vicinity of P1 and P2 promoters. Deletion analysis and in vivo monitoring of promoter activity of these promoters revealed that the three promoters have different characteristics. P1 and P2 show stress-inducible high and low promoter strengths respectively. P3 is a non-inducible moderate promoter strength. These promoters are regulated by two SOS boxes. The multiplicity of promoters and SOS boxes provides back-up systems to ensure proper regulation of recA. © 2001 Federation of European Microbiological Societies.