Publication:
Purification and characterization of a steroid-binding sialoglycoprotein from rat ventral prostate

Issued Date

1986-08-15

Resource Type

Article

ISSN

10960384
00039861

DOI

10.1016/0003-9861(86)90570-9

Other identifier(s)

2-s2.0-0022506643

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Archives of Biochemistry and Biophysics. Vol.249, No.1 (1986), 154-163

Suggested Citation

Tipaporn Limpaseni, Montri Chulavatnatol Purification and characterization of a steroid-binding sialoglycoprotein from rat ventral prostate. Archives of Biochemistry and Biophysics. Vol.249, No.1 (1986), 154-163. doi:10.1016/0003-9861(86)90570-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9667

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Purification and characterization of a steroid-binding sialoglycoprotein from rat ventral prostate

Author(s)

Tipaporn Limpaseni
 Montri Chulavatnatol

Other Contributor(s)

Mahidol University

Abstract

An androgen-dependent sialoglycoprotein was purified from the secretion of rat ventral prostate by chromatofocusing and DEAE-Sepharose column chromatography. It showed a native molecular weight of 47,000 and consisted of two dissimilar subunits with molecular weights of 20,000 and 18,000. However, each subunit contained a common peptide with molecular weight of 16,000. It also contained 442 ± 62 μg sialic acids per milligram protein and bound pregnenolone with a binding affinity of 1.2 μm -1 . Its amino acid composition was similar to those of other known prostatic steroid-binding proteins. Hence, we propose that it is the sialylated form of rat prostatic steroid-binding protein. © 1986.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/9667

Collections

Scopus 1969-1990