Publication: Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
Issued Date
2021-08-01
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ISSN
14698161
00311820
00311820
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2-s2.0-85106158049
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology. Vol.148, No.9 (2021), 1074-1082
Suggested Citation
Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria De Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel Anne Tabo, Karma G. Dolma, Veeranoot Nissapatorn Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level. Parasitology. Vol.148, No.9 (2021), 1074-1082. doi:10.1017/S0031182021000718 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/75617
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Title
Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
Other Contributor(s)
Faculty of Tropical Medicine, Mahidol University
Sikkim Manipal Institute of Medical Sciences
The University of Nottingham Malaysia Campus
De La Salle University
Walailak University
Birjand University of Medical Sciences
CICECO – Instituto de Materiais de Aveiro
Shahid Sadoughi University of Medical Sciences
University of Tabriz
University of Development Alternative
Sikkim Manipal Institute of Medical Sciences
The University of Nottingham Malaysia Campus
De La Salle University
Walailak University
Birjand University of Medical Sciences
CICECO – Instituto de Materiais de Aveiro
Shahid Sadoughi University of Medical Sciences
University of Tabriz
University of Development Alternative
Abstract
Cassia angustifolia Vahl. plant is used for many therapeutic purposes, for example, in people with constipation, skin diseases, including helminthic and parasitic infections. In our study, we demonstrated an amoebicidal activity of C. angustifolia extract against Acanthamoeba triangularis trophozoite at a micromolar level. Scanning electron microscopy (SEM) images displayed morphological changes in the Acanthamoeba trophozoite, which included the formation of pores in cell membrane and the membrane rupture. In addition to the amoebicidal activity, effects of the extract on surviving trophozoites were observed, which included cyst formation and vacuolization by a microscope and transcriptional expression of Acanthamoeba autophagy in response to the stress by quantitative polymerase chain reaction. Our data showed that the surviving trophozoites were not transformed into cysts and the trophozoite number with enlarged vacuole was not significantly different from that of untreated control. Molecular analysis data demonstrated that the mRNA expression of AcATG genes was slightly changed. Interestingly, AcATG16 decreased significantly at 12 h post treatment, which may indicate a transcriptional regulation by the extract or a balance of intracellular signalling pathways in response to the stress, whereas AcATG3 and AcATG8b remained unchanged. Altogether, these data reveal the anti-Acanthamoeba activity of C. angustifolia extract and the autophagic response in the surviving trophozoites under the plant extract pressure, along with data on the formation of cysts. These represent a promising plant for future drug development. However, further isolation and purification of an active compound and cytotoxicity against human cells are needed, including a study on the autophagic response at the protein level.
