Publication: Suppression of PmRab7 by dsRNA Inhibits WSSV or YHV Infection in Shrimp
10
Issued Date
2008-07-01
Resource Type
ISSN
14362228
Other identifier(s)
2-s2.0-44949178563
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Marine Biotechnology. Vol.10, No.4 (2008), 374-381
Suggested Citation
Chalermporn Ongvarrasopone, Mayuree Chanasakulniyom, Kallaya Sritunyalucksana, Sakol Panyim Suppression of PmRab7 by dsRNA Inhibits WSSV or YHV Infection in Shrimp. Marine Biotechnology. Vol.10, No.4 (2008), 374-381. doi:10.1007/s10126-007-9073-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18719
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Suppression of PmRab7 by dsRNA Inhibits WSSV or YHV Infection in Shrimp
Other Contributor(s)
Abstract
Viral entry into host cells requires endocytosis machineries of the host for viral replication. PmRab7, a Penaeus monodon small GTPase protein, was investigated for its function in vesicular transport during viral infection. The double-stranded RNA of Rab7 was injected into a juvenile shrimp before challenging with white spot syndrome virus (WSSV) or yellow head virus (YHV). PmRab7 mRNA was specifically decreased at 48 h after dsRNA-Rab7 injection. Silencing of PmRab7 dramatically inhibited WSSV-VP28 mRNA and protein expression. Unexpectedly, the silencing of PmRab7 also inhibited YHV replication in the YHV-infected shrimp. These results suggested that PmRab7 is a common cellular factor required for WSSV or YHV replication in shrimp. Because PmRab7 should function in the endosomal trafficking pathway, its silencing prevents successful viral trafficking necessary for replication. Silencing of PmRab7 could be a novel approach to prevent both DNA virus (WSSV) and RNA virus (YHV) infection of shrimp. © 2007 Springer Science+Business Media, LLC.